I successfully used crispr to edit mosquitoes!

[mr715]

12. Yes, it has a fully sequenced genome.

Based on work in Drosophila, the knockout mutants are viable.

This same mutation has been done in mice and they are also viable, just highly anxious and aggressive.

The interesting thing about this particular enzyme is it has two isoforms: one that generates serotonin in peripheral tissues, and one that solely operates in the nervous system. I'm knocking out the nervous system one.

The literature seems to suggest that dopamine circuits compensate for the missing serotonin, and there is enough in the somatic cells to prevent major developmental problems.